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KMID : 1007519990080020088
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Food Science and Biotechnology
1999 Volume.8 No. 2 p.88 ~ p.92
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In Vitro Antioxidant Activity of Safflower (Carthamus tinctorius L .) Seeds
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Kim, Jung Han
Oh, Won Taek/Roh, Jung Seop/Sun, Won Suck/Oh, Seung Uk/Lee, Jae Ick
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Abstract
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Antioxidant activity of the MeOH extract of unroasted and roasted safflower (Carthamus tinctorius L.) seeds was measured by their scavenging ability on the stable free radical of 1,1-diphenyl-2-picrylhydrazyl (DPPH), and their inhibitory power of ascorbic acid/Fe^(2+) and ADP/NADPH/Fe^(3+) induced lipid peroxidation in rat liver microsomes. The MeOH extract obtained from safflower seeds was fractionated with several solvents. In spite of some different spots on TLC and several different peaks on the electropherogram of capillary electrophoresis (CE) between unroasted and roasted safflower seeds, there was no significant difference on DPPH radical scavenging activities between the two. ´The EtOAc fraction exhibiting the highest DPPH radical scavenging activity was further purified by silica gel column chromatography and preparative TLC. Two DPPH radical scavengers, CT-01 and CT-02 from the EtOAc fraction and one reaction product formed from the roasting process, CT-06 from the EtOAc fraction of roasted safflower seeds, were isolated and purified using HPLC and CE. CT-Ol and CT-02 were identified as N-feruloylserotonin and N-(p-coumaroyl)serotonin and CT-06 was identified as acacetin by ¹H-NMR, ^(13)C-NMR, IR, and EI-MS. CT-01 and CT-02 showed a stronger scavenging activity by DPPH test than BHT and BHA. Antioxidant activities measured by lipid peroxidation of CT-01 and CT-02 was comparable to those of BHT and BHA, but CT-06 showed only a small amount of activity.
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KEYWORD
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